Calibration of automatic brain stereotaxic apparatus
The automatic brain stereotaxic device is an important research device in the fields of neuroanatomy, neurophysiology, neuropharmacology, and neurosurgery. Automated stereotaxic devices are widely used for accurate localization of brain injury, stimulation, and EEG recordings, and have become an important tool for studying brain structure and function.
Validation of the fully automated stereotaxic apparatus.
After the automatic stereotaxic apparatus has been moved or has not been used for a long time, it should be verified before use. Check whether the slide rule on the electrode moving frame is kept at a right angle, and use a triangular plate to measure whether the angle of the slide rule is a right angle; whether the connecting parts and screws are loose; whether the slide rule is loose; check the parallelism of the main frame arm; Symmetry, whether the small frame is parallel to the main frame.
After the automatic stereotaxic apparatus has been checked without failure, the following verification operations can be performed:
1. Loosen the ear post on both sides, slide it back and forth on the main frame, and then install it according to the original size to see if the ear bar tips on both sides are completely correct.
2. Remove one side of the ear rod, install one side of the electrode moving frame, and move the slide rule up and down, front, back, left, right, and up, so that the metal positioning needle tip installed on the electrode clip faces ZX on the tip of the ear rod, record the scale reading of each slide rule, and then Remove the mobile frame and install it, measure the tip of the ear rod according to the above method, write down the readings of the three sliders, and repeat the operation to obtain the average value. First, adjust the two slides on the automated stereotaxic apparatus according to the experimental requirements.
3. Then use a spirit level to adjust the front and rear of the two slides. horizontally. At this time, adjust the verticality of the manual micro-propeller placed on the slideway according to the above scale.
The mouse brain localization system can be roughly divided into two types:
1. Use the ear wire ZX to locate, first contact two ear sticks in the middle of the slideway of the automatic stereotaxic instrument (the readings of the two ear sticks are the same), and tighten the screws.
2. Remove one ear stick, the other ear stick does not move. Adjust the moving pusher so that the verification electrode touches the ZX point of the ear bar, that is, point A (ie, the ZX point of the interaural line), and note the scale value.
3. Move the pusher horizontally over the incisor hook and bring the incisor hook plane into contact with the verification electrode. At this time, the horizontal section between the ZX point of the external auditory canal and the upper edge of the incisor plate is set to 0 point and recorded as H0. At this time, the difference between the basic level of the anterior fontanelle and the posterior fontanelle of the animal is 0-0.1 mm. In addition, it is stipulated that the ear line above the ZX point is +, and the downward is -; the mouth side is +, and the caudal side is -.
4. Use the skull mark for positioning (commonly used bregma), that is, the bregma is the 0 point of the mouth and tail, the bregma is +, and the tail is -, and other positioning methods are the same as the above.