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Tail suspension experiment video analysis system


The video analysis system of the tail suspension experiment makes the experimental animals hang their heads downward by fixing their tails. The animals struggle desperately in this environment and try to escape but cannot escape, thus providing an unavoidable oppressive environment. After a period of experimentation, Record a series of parameters in the process of animals in this environment producing a hopeless immobility state. This typical "immobility state" exhibited by animals reflects a so-called "behavioral despair state". The behavioral despair model is similar to depression, and is sensitive to most antidepressants, and its efficacy is significantly correlated with clinical efficacy, so it is widely used in the primary selection of antidepressants.

Pre-consultation
Description Parameters Resources

The video analysis system of the tail suspension experiment makes the experimental animals hang their heads downward by fixing their tails. The animals struggle desperately in this environment and try to escape but cannot escape, thus providing an unavoidable oppressive environment. After a period of experimentation, Record a series of parameters in the process of animals in this environment producing a hopeless immobility state. This typical "immobility state" exhibited by animals reflects a so-called "behavioral despair state". The behavioral despair model is similar to depression, and is sensitive to most antidepressants, and its efficacy is significantly correlated with clinical efficacy, so it is widely used in the primary selection of antidepressants.

 

1. Hardware parameters:

1. Hardware dimensions (rat): 350×350×500mm

2. Hardware dimensions (mice): 200 × 200 × 320mm

2. Camera parameters:

1. Camera: gun camera

2. Camera needle frequency rate: 28/30 Zhen 600X, resolution: not less than 650TVL.

3. Color: Color.

4. Pixels: no less than 976*582.

5. Minimum illumination: F1.2.50IRE.

6. Camera pin frequency rate: 28/30 frames 600X, gun type is a camera

7. Camera resolution: ≥976*582, camera adapter power supply: AC24V, DC12V.

8. Camera type: 2.8~12mm automatic zoom.

3. Software parameters:

1. One-click drawing area

The labmaze center fixed-point drawing method is convenient to draw the labyrinth area required for the experiment. After the experiment, it is found that a certain area is not drawn, and this area can be temporarily added without re-analyzing the video. The software can directly obtain the indicators of this area.

2. Intelligent AI algorithm

The software automatically recognizes the volume and shape of animals by learning and extracts them from complex backgrounds. It has strong anti-interference ability and filtering ability. Even if there are many ripples that interfere with the picture, it can accurately track animals.

Tracking method: The tip of the nose, the center, and the root of the tail track three points and analyze the data corresponding to the three points at the same time, etc.


3. Diversified experimental process design

The software is openly edited for conditions such as time, area, speed, hardware, steps, events, etc., which is convenient for editing the experimental process mentioned in any literature.

4. Human-computer interaction verification

The software automatically saves the experimental process video, and the experimental video and analysis indicators can be played back simultaneously with the same window, verifying the unity of software analysis and manual calculation, and ensuring the authenticity of the analysis results.

5. Rich statistical indicators

The software can analyze more than 200 indicators such as the number of visits, stay time, percentage, moving distance, speed, rotation between regions, trajectory diagrams, time series diagrams, heat map, and event relationships in each quadrant or area;

6. GLP management

The software has the function of rights management, the administrator assigns the experimental user account and manages the user rights; reviews the operation of the experimenter

7. Strong compatibility and scalability

Strong compatibility: It can be used as a platform with similar products of other brands at home and abroad. It is convenient to expand and upgrade the platform in the future, and the secondary expansion software will no longer cost;

Strong expansibility: the software triggers electrical stimulation, gas stimulation, signal lights, horns, microinjection pumps, automatic feeders, infrared sensors, gravity sensors, pressure bars, nose probes, touch screen, diet, water, in-body multi-channel through input and output ports Equipment for electrophysiology, optogenetics, calcium signaling, etc. Realize latency-free synchronization and expand behavioral experiments to a larger dimension;

8. The software is allowed to be installed on multiple computers, the software is allowed to work on laptop computers, supports online and offline video analysis, and facilitates statistics of data indicators;

9. Data indicators: experiment time, resting time and times, struggling time and times, climbing time and times

Some published articles

1.Effects of volatile oils from Cyperus rotundus on the anxiety behaviors of mice exposed to chronic restraint stress model in rats induced by closed head impacts

2. Effects of “Shenning Zangxiang” on behavior and gabaergic pathway in insomnia rats

3. The Gut Microbiota Changed by Short-term Low-dose Ethanol Withdrawal and Related to the Anxiety Like Behavior in Male Rats

For more literature, please consult: 400-016-4066

This parameter is the basic parameter of the equipment, please contact our company for specific parameters

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