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CPP Conditioned Place Preference Video Analysis System

The CPP Conditioned Place Preference (CPP) test is a classic experimental model for evaluating drug-psychotic dependence, and it is also an effective tool widely used to find anti-drug-seeking behaviors. In this experiment, experimental animals (rats, mice) were placed in the white observation area of the conditioned place preference box, and a psychotropic drug (such as morphine) was administered, and then the experimental animals were observed in the black area and white area of the conditioned place preference box. There are small gates between the white area, the black area and the gray area for animals to shuttle freely. Each time the animals are in the dosing area, they will have a positional preference for the black and white areas under the effect of the drug's rewarding effect, and the degree of which is related to the mental dependence of the drug.

Pre-consultation
Description Parameters Resources

The CPP Conditioned Place Preference (CPP) test is a classic experimental model for evaluating drug-psychotic dependence, and it is also an effective tool widely used to find anti-drug-seeking behaviors. In this experiment, experimental animals (rats, mice) were placed in the white observation area of the conditioned place preference box, and a psychotropic drug (such as morphine) was administered, and then the experimental animals were observed in the black area and white area of the conditioned place preference box. There are small gates between the white area, the black area and the gray area for animals to shuttle freely. Each time the animals are in the dosing area, they will have a positional preference for the black and white areas under the effect of the drug's rewarding effect, and the degree of which is related to the mental dependence of the drug.

1. Hardware parameters:

1. Specifications of rat preference box: Overall size: 720× 250× 350mm / Left and right box size: 305× 250× 350mm / Middle box size: 110× 250× 350mm

2. Specifications of mouse preference box: Dimensions: 400× 150× 250mm / Left and right box size: 170× 150× 250mm / Middle box size: 60× 150× 250mm

3. Bottom tactile mode: fence, tablet, grid

2. Camera parameters:

1. Camera: HD without distortion

2. Color: color/black and white

3. Camera resolution ≥640*480

4. Camera adapter power supply: DC 12V

3. Software parameters:

1. One-click drawing area

The labmaze center fixed-point drawing method is convenient to draw the labyrinth area required for the experiment. After the experiment, it is found that a certain area is not drawn, and this area can be temporarily added without re-analyzing the video. The software can directly obtain the indicators of this area.

2. Intelligent AI algorithm

The software automatically recognizes the volume and shape of animals by learning and extracts them from complex backgrounds. It has strong anti-interference ability and filtering ability. Even if there are many ripples that interfere with the picture, it can accurately track animals.

Tracking method: The tip of the nose, the center, and the root of the tail track three points and analyze the data corresponding to the three points at the same time, etc.

3. Diversified experimental process design

The software is openly edited for conditions such as time, area, speed, hardware, steps, events, etc., which is convenient for editing the experimental process mentioned in any literature.


4. Human-computer interaction verification

The software automatically saves the experimental process video, and the experimental video and analysis indicators can be played back simultaneously with the same window, verifying the unity of software analysis and manual calculation, and ensuring the authenticity of the analysis results.

5. Rich statistical indicators

The software can analyze more than 200 indicators such as the number of visits, stay time, percentage, moving distance, speed, rotation between regions, trajectory diagrams, time series diagrams, heat map, and event relationships in each quadrant or area;

6. GLP management

The software has the function of rights management, the administrator assigns the experimental user account and manages the user rights; reviews the operation of the experimenter

7. Strong compatibility and scalability

Strong compatibility: It can be used as a platform with similar products of other brands at home and abroad. It is convenient to expand and upgrade the platform in the future, and the secondary expansion software will no longer cost;

Strong expansibility: the software triggers electrical stimulation, gas stimulation, signal lights, horns, microinjection pumps, automatic feeders, infrared sensors, gravity sensors, pressure bars, nose probes, touch screen, diet, water, in-body multi-channel through input and output ports Equipment for electrophysiology, optogenetics, calcium signaling, etc. Realize latency-free synchronization and expand behavioral experiments to a larger dimension;

8. The software is allowed to be installed on multiple computers, the software is allowed to work on laptop computers, supports online and offline video analysis, and facilitates statistics of data indicators;

9. Data indicators: observation time\shuttle times\left box activity time\middle box activity time\right box activity time\left box activity distance\middle box activity distance\right box activity distance\total activity distance, etc.

Some published articles

1. Original Article Downregulation of Sirtuin1 in the rat nucleus accumbens was beneficial for the alleviation of heroin addiction

2. SIRT1 regulates Synaptic plasticity nucleus accumbens in heroin-addicted rats

3.Effects of adenosine receptor regulation on behavior of propofol-dependent rats

For more literature, please consult: 400-016-4066

This parameter is the basic parameter of the equipment, please contact our company for specific parameters

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