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T maze video analysis system

Description Parameters Resources

Experimental Principles

Nearly half a century ago, Kivy and Dember et al. demonstrated that rats can distinguish changes in the color of the arms of a T-maze. They found that male rats were placed in the main arms of the T-maze for 15-30 minutes, allowing them to see but not enter the black and white arms. Then, change the color of one of the arms so that both arms are black or white. Allow rats to freely choose their T-arms. The results showed that rats always chose the arm that changed color (the new arm). This process relies on the memory of animals to complete. The T-maze experiment developed from this has become one of the most commonly used animal models for evaluating spatial memory. Of course, the current T-maze uses food rather than arm color as the driving force for animal exploration. This model is usually used to study the spatial Working memory of animals, that is, to determine the information that is useful only during the current operation. The improved T-maze can also be used to evaluate reference memory, which records useful information for any day or test in this experiment. T maze is a classical behavioral method to detect spatial Working memory of rodents, which is related to prefrontal cortex function.

Technical parameters:

1. Total experimental time

2. Total itinerary of the event

3. Left arm entry times

4. Number of Right Arm Entries

5. Left arm latency (time from main arm to left arm)

6. Right arm latency (time from main arm to right arm)

7. Main boom dwell time

8. The range of main arm activity: 1) There are multiple geometric shapes to choose from in the open field, which can easily outline various shapes of the open field; 2) Supports offline and online real-time analysis, saving experimental time and personnel; 3) The software automatically tracks and analyzes, generates EXCEL files, and can be directly analyzed in statistical analysis software such as SPSS; (The software also comes with powerful analysis functions, including over 30 types of statistical tests) 4) It can manually assist in recording up to 26 phenomena that cannot be detected by the video software, such as standing times, grooming times, fecal particles, jumping times, etc. 5) The video collection has original recordings to ensure the traceability of the test; (The software can choose to save the original recording or not to save the recording to save hard disk space) 6) The experimental process is automated, avoiding subjective errors caused by manual observation and counting, as well as interference with experimental animals; Experimental method: 2 days before training, touch the mice and place them in a T-maze for 10 minutes. Place food in the two arm bowls of the maze and remove them after the mice have finished feeding.

Hardware size:

Rat: Arm length 50cm, arm width 10cm, arm height 30cm, bracket height 50cm

Mouse: Arm length 30cm, arm width 6cm, arm height 20cm, bracket height 50cm

Material: aluminum profile and acrylic

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